Fast Protein Liquid Chromatography (FPLC) is a technique used in biochemistry and molecular biology for separating, purifying, and analyzing proteins and other biomolecules. This method uses a liquid mobile phase and a solid stationary phase to separate proteins based on their size, charge, hydrophobicity, or affinity for specific ligands. FPLC offers rapid and efficient protein purification, making it essential for research and pharmaceutical applications.